Rosendal E, Byström JW, Gröning R, Gwon YD, Nilsson E, Sharma A, Espaillat A, Hanke L, Thunberg T, McInerney G, Puhar A, Cava F, Hedestam GBK, Monsen T, Elgh F, Blomkvist B, Marklund I, Ahlm C, Evander M, Normark J, Johansson A, Överby AK, Forsell MN
medRxiv - (-) - [2020-06-04; online 2020-06-04]
To assess the current coronavirus pandemic, there is a pressing need to determine the exposure and seroconversion to SARS-CoV-2 on a local and global level. Here, we demonstrate a sensitive and specific S-protein based assay that is well suited for detection of weak SARS- CoV-2-directed IgG responses, and that could identify exposed individuals with asymptomatic infection without the requirement of PCR diagnostics. Our results raise the possibility that on- going population-based studies using less sensitive state-of-the-art serological assays may significantly underestimate the frequency of exposure and seroconversion to SARS-CoV-2.