Organotypic human ex vivo models for coronavirus disease 2019 research and drug development.

Youhanna S, Wright SC, Lauschke VM

Curr Opin Pharmacol 59 (-) 11-18 [2021-04-27; online 2021-04-27]

Since the discovery of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in late 2019, intense research efforts on an unprecedented scale have focused on the study of viral entry mechanisms and adaptive immunity. While the identification of angiotensin-converting enzyme 2 (ACE2) and other co-receptors has elucidated the molecular and structural basis for viral entry, the pathobiological mechanisms of SARS-CoV-2 in human tissues are less understood. Recent advances in bioengineering have opened opportunities for the use of organotypic human tissue models to investigate host-virus interactions and test antiviral drug candidates in a physiological context. Although it is too early to accurately quantify the added value of these systems compared with conventional cell systems, it can be assumed that these advanced three-dimensional (3D) models contribute toward improved result translation. This mini-review summarizes recent work to study SARS-CoV-2 infection in human 3D tissue models with an emphasis on the pharmacological tools that have been developed to understand and prevent viral entry and replication.

Type: Review

PubMed 34029832

DOI 10.1016/j.coph.2021.04.006

Crossref 10.1016/j.coph.2021.04.006

pii: S1471-4892(21)00050-3
pmc: PMC8075816

Publications 9.5.0