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for updates. The development team can be contacted at datacentre@scilifelab.se
if you have any question.Perez-Zabaleta M, Archer A, Khatami K, Jafferali MH, Nandy P, Atasoy M, Birgersson M, Williams C, Cetecioglu Z
Sci Total Environ 858 (Pt 3) 160023 [2022-11-08; online 2022-11-08]
Wastewater-based epidemiology (WBE) can be used to track the spread of SARS-CoV-2 in a population. This study presents the learning outcomes from over two-year long monitoring of SARS-CoV-2 in Stockholm, Sweden. The three main wastewater treatment plants in Stockholm, with a total of six inlets, were monitored from April 2020 until June 2022 (in total 600 samples). This spans five major SARS-CoV-2 waves, where WBE data provided early warning signals for each wave. Further, the measured SARS-CoV-2 content in the wastewater correlated significantly with the level of positive COVID-19 tests (r = 0.86; p << 0.0001) measured by widespread testing of the population. Moreover, as a proof-of-concept, six SARS-CoV-2 variants of concern were monitored using hpPCR assay, demonstrating that variants can be traced through wastewater monitoring. During this long-term surveillance, two sampling protocols, two RNA concentration/extraction methods, two calculation approaches, and normalization to the RNA virus Pepper mild mottle virus (PMMoV) were evaluated. In addition, a study of storage conditions was performed, demonstrating that the decay of viral RNA was significantly reduced upon the addition of glycerol to the wastewater before storage at -80 °C. Our results provide valuable information that can facilitate the incorporation of WBE as a prediction tool for possible future outbreaks of SARS-CoV-2 and preparations for future pandemics.
Funder: KAW/SciLifeLab National COVID program
Research Area: Environmental virus profiling
PubMed 36356735
DOI 10.1016/j.scitotenv.2022.160023
Crossref 10.1016/j.scitotenv.2022.160023
pmc: PMC9640212
pii: S0048-9697(22)07123-6