SARS-CoV-2 spike protein aggregation is triggered by bacterial lipopolysaccharide.

Petrlova J, Samsudin F, Bond PJ, Schmidtchen A

FEBS Lett 596 (19) 2566-2575 [2022-10-00; online 2022-09-11]

SARS-CoV-2 spike (S) protein is crucial for virus invasion in COVID-19. Here, we showed that lipopolysaccharide (LPS) can trigger S protein aggregation at high doses of LPS and S protein. We demonstrated the formation of S protein aggregates by microscopy analyses, aggregation and gel shift assays. LPS at high levels boosts the formation of S protein aggregates as detected by amytracker and thioflavin T dyes that specifically bind to aggregating proteins. We validated the role of LPS by blocking the formation of aggregates by the endotoxin-scavenging thrombin-derived peptide TCP-25. Aggregation-prone sequences in S protein are predicted to be nearby LPS binding sites, while molecular simulations showed stable formation of S protein-LPS higher-order oligomers. Collectively, our results provide evidence of LPS-induced S protein aggregation.

Category: Biochemistry

Category: Health

Funder: VR

Type: Journal article

PubMed 36050806

DOI 10.1002/1873-3468.14490

Crossref 10.1002/1873-3468.14490

pmc: PMC9538650

Publications 9.5.0