Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing.
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Mancini F, Barbanti F, Scaturro M, Errico G, Iacobino A, Bella A, Riccardo F, Marsili G, Stefanelli P, Pezzotti P, Rezza G, Ciervo A, on behalf ISS COVID-19 study group
Emerg Microbes Infect 9 (1) 1393-1396 [2020-12-00; online 2020-06-20]
The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
PubMed 32552549
DOI 10.1080/22221751.2020.1775500
Crossref 10.1080/22221751.2020.1775500