Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing.

Mancini F, Barbanti F, Scaturro M, Errico G, Iacobino A, Bella A, Riccardo F, Marsili G, Stefanelli P, Pezzotti P, Rezza G, Ciervo A, on behalf ISS COVID-19 study group

Emerg Microbes Infect 9 (1) 1393-1396 [2020-12-00; online 2020-06-20]

The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

Category: Other

Type: Journal article

PubMed 32552549

DOI 10.1080/22221751.2020.1775500

Crossref 10.1080/22221751.2020.1775500

pmc: PMC7473159

Publications 9.2.2