A Multiparametric and High-Throughput Platform for Host-Virus Binding Screens.

Schlegel J, Porebski B, Andronico L, Hanke L, Edwards S, Brismar H, Murrell B, McInerney GM, Fernandez-Capetillo O, Sezgin E

Nano Lett 23 (9) 3701-3707 [2023-05-10; online 2023-03-09]

Speed is key during infectious disease outbreaks. It is essential, for example, to identify critical host binding factors to pathogens as fast as possible. The complexity of host plasma membrane is often a limiting factor hindering fast and accurate determination of host binding factors as well as high-throughput screening for neutralizing antimicrobial drug targets. Here, we describe a multiparametric and high-throughput platform tackling this bottleneck and enabling fast screens for host binding factors as well as new antiviral drug targets. The sensitivity and robustness of our platform were validated by blocking SARS-CoV-2 particles with nanobodies and IgGs from human serum samples.

Category: Biochemistry

Category: Health

Funder: H2020

Funder: KAW/SciLifeLab

Funder: VR

Type: Journal article

PubMed 36892970

DOI 10.1021/acs.nanolett.2c04884

Crossref 10.1021/acs.nanolett.2c04884

pmc: PMC10176574

Publications 9.5.0