Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination.

Wu S, Liu X, Ye S, Liu J, Zheng W, Dong X, Yin X

Heliyon - (-) e06886 [2021-04-21; online 2021-04-21]

RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19 1,2, which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.

Category: Genomics & transcriptomics

Category: Other

Type: Journal article

PubMed 33903853

DOI 10.1016/j.heliyon.2021.e06886

Crossref 10.1016/j.heliyon.2021.e06886

pii: S2405-8440(21)00989-0
pmc: PMC8059943

Publications 9.5.0